JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Genetics
/
一种快速的银染色协议, 能够使用非变性聚丙烯酰胺凝胶对 SSR 标记进行简单而有效的检测
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Genetics
A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel
Please note that all translations are automatically generated.
Click here for the English version.
一种快速的银染色协议, 能够使用非变性聚丙烯酰胺凝胶对 SSR 标记进行简单而有效的检测
DOI:
10.3791/57192-v
•
10:27 min
•
April 20, 2018
•
Ling Huang*
1
,
Xiaohui Deng*
1
,
Ronghua Li
,
Yanshi Xia
,
Guihua Bai
,
Kadambot H.M. Siddique
,
Peiguo Guo
1
College of Life Sciences
,
Guangzhou University
,
2
Hard Winter Wheat Genetics Research Unit
,
United States Department of Agriculture – Agricultural Research Service
,
3
The UWA Institute of Agriculture
,
University of Western Australia
Chapters
00:04
Title
01:38
Preparation of Polyacrylamide Gels for Electrophoresis
04:03
Running Gels and Silver Staining for Detecting SSR Markers in a Non-polyacrylamide Gel
07:43
Results: Fast Silver Staining to Detect SSR Markers on a Non-denaturing Polyacrylamide Gel
09:18
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
在这里, 我们报告一个简单和低成本的银染色协议, 只需三试剂和7分钟的处理, 并适合快速生成高质量的 SSR 数据的遗传分析。
Tags
Silver Staining
SSR Markers
Non-denaturing Polyacrylamide Gel
Genetic Research
Marker-assisted Selection
Molecular Breeding
Rapid Genotyping
Easy
Low Cost
Optimized Protocol
Bind-silane
Repel-silane
TEMED
APS
Electrophoresis
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
单细胞测序拷贝数改变的检测
使用生物素化补骨脂素全球定位RNA-RNA相互作用
用荧光PCR毛细管电泳技术在基因型CRISPR / Cas9介导的敲除突变体的高通量形式
激光 Microirradiation 研究
体内
细胞对简单复杂 DNA 损伤的反应
一种基于阵列的比较基因组杂交平台, 有效检测快中子诱导的
苜蓿蒺藜
突变体的拷贝数变化
斑马斑马
模型系统中 CRISPR/Cas9-generated 基因挖空的高效生产与鉴定
CRISPR/Cas9 对小鼠和人造血祖细胞的高效基因破坏
慢病毒载体介导的转基因小鼠生产: 一种简单高效的创始人直接研究方法
一种快速、定量的后向基因组转化和变异的多肽映射方法
用小鼠脾评估病原体相关分子模式对时钟基因表达的影响
Read Article