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Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies
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Developmental Biology
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JoVE Journal Developmental Biology
Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies

Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies

DOI:
10.3791/57212-v

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07:18 min

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April 19, 2018

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, , , ,
1Laboratory of Muscle Stem Cells and Gene Regulation,National Institutes of Health, 2Office of Science and Technology, National Institute of Arthritis, Musculoskeletal and Skin Diseases (NIAMS),National Institutes of Health

Chapters

  • 00:05Title
  • 00:41Cryo-embedding of the Tibialis Anterior Muscle
  • 01:46Cryostat Sectioning
  • 02:33Immunofluorescence and Staining
  • 05:27Results: Visualization of Satellite Cells in Adult Resting Muscle Sections is Improved Using Immunofluorescence-based Staining
  • 06:40Conclusion

Summary

Automatic Translation

Automatic Translation

The precise identification of satellite cells is essential for studying their functions under various physiological and pathological conditions. This article presents a protocol to identify satellite cells on adult skeletal muscle sections by immunofluorescence-based staining.

Tags

Skeletal MuscleSatellite CellsImmunofluorescencePax7LamininCryosectioningCryostatAntigen RetrievalImmunostaining

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