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Developmental Biology
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人成体和胎心标本中原发性心外膜细胞的分离培养
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
The Isolation and Culture of Primary Epicardial Cells Derived from Human Adult and Fetal Heart Specimens
Please note that all translations are automatically generated.
Click here for the English version.
人成体和胎心标本中原发性心外膜细胞的分离培养
DOI:
10.3791/57370-v
•
08:57 min
•
April 24, 2018
•
Esther Dronkers
,
Asja T. Moerkamp
,
Tessa van Herwaarden
,
Marie-José Goumans
,
Anke M. Smits
1
Department of Molecular Cell Biology
,
Leiden University Medical Center
Chapters
00:04
Title
00:51
Dissection of Epicardial Layer and Isolation of Epicardial Derived Cells
04:10
Culture of EPDCs
05:32
Induction of Epithelial to Mesenchymal Transition in EPDCs
06:31
Results: Isolation of EPDCs from Human Adult Cardiac Tissue and Induction and Validation of EMT in EPDCs
08:17
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
心外膜在心脏的发育和修复中起着至关重要的作用, 它为心肌壁提供细胞和生长因子。在这里, 我们描述了一种培养人原发性心外膜细胞的方法, 能够对它们的发育和成人特征进行研究和比较。
Tags
Epicardial Cells
Primary Cells
Cell Culture
Epithelial-mesenchymal Transition
Cell Isolation
Trypsin Dissociation
Cell Suspension
Cell Strainer
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