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Developmental Biology
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人間の成人および胎児心標本由来分離とプライマリの心外膜細胞の培養
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
The Isolation and Culture of Primary Epicardial Cells Derived from Human Adult and Fetal Heart Specimens
Please note that all translations are automatically generated.
Click here for the English version.
人間の成人および胎児心標本由来分離とプライマリの心外膜細胞の培養
DOI:
10.3791/57370-v
•
08:57 min
•
April 24, 2018
•
Esther Dronkers
,
Asja T. Moerkamp
,
Tessa van Herwaarden
,
Marie-José Goumans
,
Anke M. Smits
1
Department of Molecular Cell Biology
,
Leiden University Medical Center
Chapters
00:04
Title
00:51
Dissection of Epicardial Layer and Isolation of Epicardial Derived Cells
04:10
Culture of EPDCs
05:32
Induction of Epithelial to Mesenchymal Transition in EPDCs
06:31
Results: Isolation of EPDCs from Human Adult Cardiac Tissue and Induction and Validation of EMT in EPDCs
08:17
Conclusion
Summary
Automatic Translation
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Automatic Translation
心外膜細胞と心筋壁に成長因子を提供することによって開発し、中心部の修復に重要な役割を果たしています。ここでは、研究と彼らの発達と大人の特性の比較を可能にする培養ひと心外膜細胞に手法について述べる。
Tags
Epicardial Cells
Primary Cells
Cell Culture
Epithelial-mesenchymal Transition
Cell Isolation
Trypsin Dissociation
Cell Suspension
Cell Strainer
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