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小鼠致耐受性树突状细胞的发育及功能表征
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JoVE Journal Medicine
Development and Functional Characterization of Murine Tolerogenic Dendritic Cells
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小鼠致耐受性树突状细胞的发育及功能表征

DOI:
10.3791/57637-v

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09:51 min

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May 18, 2018

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, ,
1Department of Biochemistry, School of Medicine,Case Western Reserve University, 2Department of Pediatrics, Division of Pediatric Hematology/Oncology,Case Western Reserve University, 3Angie Fowler Cancer Institute, Rainbow Babies & Children’s Hospital,University Hospitals, Cleveland

Chapters

  • 00:04Title
  • 01:05Bone Marrow-derived Dendritic Cell (BMDC) Preparation
  • 03:25T Cell Syngeneic Proliferation Assay
  • 07:26Results: Representative Tolerogenic DC (TolDC) Analyses
  • 08:53Conclusion

Summary

Automatic Translation

Automatic Translation

在这里, 我们提出了一个协议, 开发和表征致耐受性树突状细胞 (TolDCs), 并评估其免疫治疗效用。

Tags

MurineTolerogenicDendritic CellsAutoimmune DisordersMultiple SclerosisImmunotherapyCell DevelopmentFunctional CharacterizationC57BL/6 MiceBone MarrowGM-CSFIL-4Cell CultureCell HarvestingCell LysisCell FilteringCell PlatingCell FeedingCell Incubation

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