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Journal
/
Genetics
/
CRISPR/Cas9 基因编辑制作人类疟疾寄生虫的条件突变体
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JoVE Journal
Genetics
CRISPR/Cas9 Gene Editing to Make Conditional Mutants of Human Malaria Parasite
P. falciparum
Please note that all translations are automatically generated.
Click here for the English version.
CRISPR/Cas9 基因编辑制作人类疟疾寄生虫的条件突变体
DOI:
10.3791/57747-v
•
09:25 min
•
September 18, 2018
•
Heather M. Kudyba*
1,2
,
David W. Cobb*
1
,
Anat Florentin
2
,
Michelle Krakowiak
,
Vasant Muralidharan
2
1
Department of Cellular Biology
,
University of Georgia
,
2
Center for Tropical and Emerging Global Diseases
,
University of Georgia
Chapters
00:04
Title
00:30
Choosing a gRNA Sequence
01:31
Precipitating DNA for Transfection
03:00
Transfecting RBCs with the CRISPR/Cas9 Plasmids
06:49
Cloning Parasites by Limiting Dilution
08:16
Results: Tagging and Conditional Knockdown of PfHsp70x
09:01
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一种利用 CRISPR/Cas9 基因组编辑在
恶性疟原虫
中产生
glmS
的条件击倒突变体的方法。
Tags
CRISPR/Cas9
Gene Editing
Conditional Mutants
Plasmodium Falciparum
Malaria Parasite
Protein Function
Knockdown
GRNA
Transfection
RBCs
Cytomix Buffer
RPMI
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