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Biochemistry

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JoVE Journal Biochemistry
Purificazione efficiente e sviluppo di analisi LC-MS/MS-based per dieci-undici traslocazione-2 5-metilcitosina diossigenasi
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Journal (Biochemistry)
High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis (QDB)
 
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Purificazione efficiente e sviluppo di analisi LC-MS/MS-based per dieci-undici traslocazione-2 5-metilcitosina diossigenasi

Article DOI: 10.3791/57798-v • 10:34 min • October 15th, 2018
October 15th, 2018 •
*1, *1, *1, 1, 1
1Division of Pharmaceutical Sciences, School of Pharmacy, University of Missouri-Kansas City
* These authors contributed equally

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Qui, presentiamo un protocollo per una purificazione efficiente unico passaggio dell'essere umano senza tag attivo dieci-undici traslocazione-2 (TET2) 5-metilcitosina diossigenasi usando la cromatografia a scambio ionico e sua analisi utilizzando una massa liquida di cromatografia-tandem spettrometria (LC-MS/MS)-approccio basato.

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Biochimica numero 140 dieci-undici demetilasi leucemia traslocazione diossigenasi LC-MS/MS epigenetica regolazione della trascrizione
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