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Biochemistry

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JoVE Journal Biochemistry
Effektiv rening och LC-MS/MS-baserad analys utveckling för tio-elva translokation-2 5-metylcytosin dioxygenas
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Effektiv rening och LC-MS/MS-baserad analys utveckling för tio-elva translokation-2 5-metylcytosin dioxygenas

Article DOI: 10.3791/57798-v • 10:34 min • October 15th, 2018
October 15th, 2018 •
*1, *1, *1, 1, 1
1Division of Pharmaceutical Sciences, School of Pharmacy, University of Missouri-Kansas City
* These authors contributed equally

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Här presenterar vi ett protokoll för en effektiv enda steg rening av aktiva otaggade människa tio-elva translokation-2 (TET2) 5-metylcytosin dioxygenas använder jonbyteskromatografi- och dess analys med hjälp av en flytande kromatografi-tandem mass masspektrometri (LC-MS/MS)-baserat tillvägagångssätt.

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Biokemi fråga 140 tio-elva translokation Demethylase leukemi dioxygenas LC-MS/MS epigenetik transkription reglering
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