Journal
/
Medicine
/
In Vivo Photolabeling of Cells in the Colon to Assess Migratory Potential of Hematopoietic Cells in Neonatal Mice
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
In Vivo Photolabeling of Cells in the Colon to Assess Migratory Potential of Hematopoietic Cells in Neonatal Mice

In Vivo Photolabeling of Cells in the Colon to Assess Migratory Potential of Hematopoietic Cells in Neonatal Mice

DOI:
10.3791/57929-v

•

08:39 min

•

August 10, 2018

•

, ,
1Center for Computational and Integrative Biology,Massachusetts General Hospital, 2Mucosal Immunology and Biology Research Center,Massachusetts General Hospital

Chapters

  • 00:04Title
  • 00:39Colon Cell Photoconversion
  • 03:00Intestinal Lymphocytes Isolation
  • 06:49Results: Migratory Potential of Photolabeled Intestinal Cells
  • 07:56Conclusion

Summary

Automatic Translation

Automatic Translation

The protocol described here utilizes a photolabeling approach in newborn mice to specifically identify immune cells that emigrate from the colon to extra-intestinal sites. This strategy will be useful to study host-microbiome interactions in early life.

Tags

Keywords: In Vivo PhotolabelingMucosal ImmunologyNeonatal MiceHematopoietic Cell MigrationColon Resident CellsOptogeneticsFiberoptic CannulaUV Light ExposureIntestinal Lymphocytes

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/57929?article_type=v

A Strategy to Identify de Novo Mutations in Common Disorders such as Autism and Schizophrenia
check_url/57929?article_type=v

In vivo 19F MRI for Cell Tracking
check_url/57929?article_type=v

Carotid Artery Infusions for Pharmacokinetic and Pharmacodynamic Analysis of Taxanes in Mice
check_url/57929?article_type=v

Intrauterine Telemetry to Measure Mouse Contractile Pressure In Vivo
check_url/57929?article_type=v

In Vivo Imaging and Tracking of Technetium-99m Labeled Bone Marrow Mesenchymal Stem Cells in Equine Tendinopathy
check_url/57929?article_type=v

Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies
check_url/57929?article_type=v

Injection of Syngeneic Murine Melanoma Cells to Determine Their Metastatic Potential in the Lungs
check_url/57929?article_type=v

Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells
check_url/57929?article_type=v

Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery
check_url/57929?article_type=v

In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice

Read Article