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Digital PCR para cuantificar la circulación MicroRNAs en infarto agudo de miocardio y enfermedades cardiovasculares
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Digital PCR para cuantificar la circulación MicroRNAs en infarto agudo de miocardio y enfermedades cardiovasculares

Article DOI: 10.3791/57950-v • 04:41 min • July 3rd, 2018
July 3rd, 2018 •
1, 1,2, 3, 1, 1, 1, 1, 1,4, 1
1Department of Cardiology and Angiology I, Heart Center Freiburg University, Faculty of Medicine, University of Freiburg, 2Department of Medicine, Monash University, 3Department of Medicine I, Lighthouse Core Facility, Medical Center, University of Freiburg, 4Department of Cardiology, Augustinerinnen Hospital, Academic Teaching Hospital, University of Cologne

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MicroRNAs circulación han demostrado promesa como biomarcadores para enfermedades cardiovasculares y los infartos agudos de miocardio. En este estudio, se describe un protocolo para la extracción de miRNA, transcripción inversa y PCR digital para la cuantificación absoluta de miRNAs en el suero de pacientes con enfermedad cardiovascular.

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Medicina número 137 infarto de miocardio enfermedad cardiovascular PCR digital microRNAs enfermedad coronaria biomarcadores de suero
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