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代谢综合征心力衰竭模型大鼠心房心肌细胞的分离与保留射血分数的关系
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JoVE Journal Medicine
Isolation of Atrial Cardiomyocytes from a Rat Model of Metabolic Syndrome-related Heart Failure with Preserved Ejection Fraction
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代谢综合征心力衰竭模型大鼠心房心肌细胞的分离与保留射血分数的关系

DOI:
10.3791/57953-v

•

08:31 min

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July 26, 2018

•

, , , ,
1Department of Internal Medicine and Cardiology,Charité University Medicine, 2German Center for Cardiovascular Research (DZHK)

Chapters

  • 00:04Title
  • 00:33Preparation of the Rat Heart
  • 01:35Cannulation
  • 02:50Pressure Manipulation and Digestion
  • 04:25Cell Processing and Calcium Re-adaptation
  • 05:57Functional Evaluation of Excitation-contraction-coupling
  • 07:32Results: Staining of Rat ACMs with Fluo4-AM
  • 08:02Conclusion

Summary

Automatic Translation

Automatic Translation

在这里, 我们描述了一个优化的, Langendorff 的程序, 从一个大鼠模型的代谢综合征心力衰竭与保存的弹射分数分离的单细胞心房心肌细胞。对心脏腔腔内压进行人工调节, 以产生功能完整的心肌细胞, 适合于励磁收缩耦合研究。

Tags

Atrial CardiomyocytesRat ModelMetabolic SyndromeHeart FailurePreserved Ejection FractionLangendorff ApparatusCannulationCardiomyocyte IsolationExcitation-contraction-coupling

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