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Visualisera samspelet mellan Qdot-märkt Protein och anläggningsvis modifierade λ DNA på nivån enda molekyl
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Visualisera samspelet mellan Qdot-märkt Protein och anläggningsvis modifierade λ DNA på nivån enda molekyl

Article DOI: 10.3791/57967-v • 08:56 min • July 17th, 2018
July 17th, 2018 •
*1,2, *1, 1,2, 1,2
1State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, 2Savaid Medical School, University of Chinese Academy of Sciences
* These authors contributed equally

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Här presenterar vi ett protokoll för att studera DNA-protein interaktioner av totalreflexion fluorescensmikroskopi (TIRFM) med hjälp av ett anläggningsvis modifierade λ DNA-substrat och en Quantum-dot märkt protein.

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Biologi fråga 137 enda molekyl imaging DNA-protein interaktioner fluorescerande sonder Quantum dot totalreflexion fluorescensmikroskopi λ DNA platsspecifika modifiering
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