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Qdot 标记蛋白与现场特异修饰的λ DNA 在单分子水平上的相互作用可视化
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JoVE Journal
Biology
Visualizing the Interaction Between the Qdot-labeled Protein and Site-specifically Modified λ DNA at the Single Molecule Level
Please note that all translations are automatically generated.
Click here for the English version.
Qdot 标记蛋白与现场特异修饰的λ DNA 在单分子水平上的相互作用可视化
DOI:
10.3791/57967-v
•
08:56 min
•
July 17, 2018
•
Huijun Xue*
1,2
,
Zhengyan Zhan*
1
,
Kaining Zhang
2
,
Yu Vincent Fu
2
1
State Key Laboratory of Microbial Resources, Institute of Microbiology
,
Chinese Academy of Sciences
,
2
Savaid Medical School
,
University of Chinese Academy of Sciences
Chapters
00:04
Title
00:41
Coverslip Cleaning and Functionalization
03:22
Flow Cell Assembly
04:45
Single Molecule Visualization
07:20
Results: Qdot
705
-labeled ORC Binding on λ-ARS317
08:04
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一个协议, 研究 DNA 蛋白相互作用的全内反射荧光显微镜 (TIRFM) 使用一个网站专门修改的λ DNA 基底和量子点标记蛋白。
Tags
Single Molecule Imaging
DNA-protein Interactions
Lambda DNA
Coverslip Functionalization
PEG Solution
Biotinylated Coverslip
DNA Replication
Gene Repair
Chromosome Structure
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