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Fabrication and Operation of an Oxygen Insert for Adherent Cellular Cultures
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Biology
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Fabrication and Operation of an Oxygen Insert for Adherent Cellular Cultures

Fabrication and Operation of an Oxygen Insert for Adherent Cellular Cultures

DOI:
10.3791/1695-v

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11:56 min

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January 06, 2010

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, ,
1Bioengineering,University of Illinois

Chapters

  • 00:00Title
  • 00:49Introduction
  • 01:21Material Fabrication and Assembly of the Oxygenating Insert
  • 03:57Validation of Oxygenating Insert- Calibration, Heterogeneity and Equilibration.
  • 07:45Assembly of Oxygenating Insert for Wound Healing Cell Culture
  • 09:56Validating and Using an Oxygenating Insert in Monolayer Wound Healing
  • 10:59Conclusion

Summary

Automatic Translation

Automatic Translation

Fabrication and validation of an add-on platform that offers enhanced control over the spatial and temporal oxygenation in a 6-well plate. The device is adaptable to a number of culture systems and can be used to investigate the effects of oxygen on wound healing.

Tags

FabricationOperationOxygen InsertAdherent Cellular CulturesOxygen ModulationBiomedical ResearchHypoxic ChamberTemporal ControlSpatial ControlPhysiological PhenomenaSpecialized KnowledgeEquipmentMicrofabricated InsertMultiwell PlatesPolydimethylsiloxane InsertPassive Microfluidic Gas NetworkGas-permeable MembraneOxygen DeliveryGas CylindersFabrication TechniquesSU-8 PhotolithographyReplica MoldingPDMS SpinningSilicon Wafer

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