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Visualizing the Interaction Between the Qdot-labeled Protein and Site-specifically Modified λ DNA at the Single Molecule Level
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JoVE Journal Biology
Visualizing the Interaction Between the Qdot-labeled Protein and Site-specifically Modified λ DNA at the Single Molecule Level

Visualizing the Interaction Between the Qdot-labeled Protein and Site-specifically Modified λ DNA at the Single Molecule Level

DOI:
10.3791/57967-v

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08:56 min

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July 17, 2018

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, , ,
1State Key Laboratory of Microbial Resources, Institute of Microbiology,Chinese Academy of Sciences, 2Savaid Medical School,University of Chinese Academy of Sciences

Chapters

  • 00:04Title
  • 00:41Coverslip Cleaning and Functionalization
  • 03:22Flow Cell Assembly
  • 04:45Single Molecule Visualization
  • 07:20Results: Qdot705-labeled ORC Binding on λ-ARS317
  • 08:04Conclusion

Summary

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Here, we present a protocol to study DNA-protein interactions by total internal reflection fluorescence microscopy (TIRFM) using a site-specifically modified λ DNA substrate and a Quantum-dot labeled protein.

Tags

Keywords: Single Molecule ImagingDNA-protein InteractionsLambda DNACoverslip FunctionalizationPEG SolutionBiotinylated CoverslipDNA ReplicationGene RepairChromosome Structure

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