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Journal / Cancer Research / Phosphopeptide anrikning tillsammans med…
JoVE Journal
Cancer Research

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JoVE Journal Cancer Research
Phosphopeptide anrikning tillsammans med etikett-fri kvantitativa masspektrometri att undersöka Phosphoproteome vid prostatacancer
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Phosphopeptide anrikning tillsammans med etikett-fri kvantitativa masspektrometri att undersöka Phosphoproteome vid prostatacancer

Article DOI: 10.3791/57996-v • 12:23 min • August 2nd, 2018
August 2nd, 2018 •
*1,2, *3, 4, 5, 1,2,3,6,7
1Graduate Program in Cellular and Molecular Pharmacology, School of Graduate Studies, Rutgers University, The State University of New Jersey, 2Graduate Program in Quantitative Biomedicine, School of Graduate Studies, Rutgers University, The State University of New Jersey, 3Department of Medicine, Division of Medical Oncology, Rutgers Robert Wood Johnson Medical School, 4Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, Jonsson Comprehensive Cancer Center, UCLA Metabolomics Center, and California NanoSystems Institute, David Geffen School of Medicine, University of California, Los Angeles, 5Mork Family Department of Chemical Engineering and Materials Science, University of Southern California, 6Pharmacology, Rutgers Robert Wood Johnson Medical School, 7Cancer Metabolism and Growth Program, Rutgers Cancer Institute of New Jersey
* These authors contributed equally

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Det här protokollet beskriver en procedur för att extrahera och berika phosphopeptides från prostata cancer cellinjer eller vävnader för en analys av den phosphoproteome via mass spectrometry-baserad proteomik.

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Cancerforskning fråga 138 prostatacancer masspektrometri proteomik phosphoproteomics fosforylering kinaser cellsignalering
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