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快速、安全、简便的人工床边核酸提取法检测全血标本中的病毒
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Immunology and Infection
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JoVE Journal Immunology and Infection
Rapid, Safe, and Simple Manual Bedside Nucleic Acid Extraction for the Detection of Virus in Whole Blood Samples
Please note that all translations are automatically generated. Click here for the English version.

快速、安全、简便的人工床边核酸提取法检测全血标本中的病毒

DOI:
10.3791/58001-v

•

06:59 min

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June 30, 2018

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, ,
1Virus Research & Development Laboratory,Statens Serum Institut, 2Infectious Disease Research Unit,University of Southern Denmark

Chapters

  • 00:04Title
  • 00:44Preparation for Virus Inactivation and Rapid Nucleic Acid Extraction
  • 02:02Blood Collection from Patients with Signs and Symptoms of a Virus Infection
  • 02:50Simplified Nucleic Acid Extraction from Whole Blood
  • 05:04Results: Sensitivity of the Simplified NA Extraction Method
  • 06:22Conclusion

Summary

Automatic Translation

Automatic Translation

在这里, 我们提出了一个快速病毒核酸提取从病毒灭活全血的协议。萃取是直接在采血管, 不需要设备或电力。该方法不依赖于实验室设施, 可以在任何地方使用 (例如在野战医院)。

Tags

Manual Bedside Nucleic Acid ExtractionVirus DetectionWhole Blood SamplesPoint-of-care DiagnosticsBlood CollectionMagnetic Glass ParticlesWash BuffersElution BufferVirus InactivationNucleic Acid Purification

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