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SA-β-加拉基沙酶筛查检测,用于识别治疗药物
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SA-β-Galactosidase-Based Screening Assay for the Identification of Senotherapeutic Drugs
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SA-β-加拉基沙酶筛查检测,用于识别治疗药物

DOI:
10.3791/58133-v

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07:39 min

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June 28, 2019

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, , , , ,
1Department of Molecular Medicine and the Center on Aging,The Scripps Research Institute, 2Department of Biochemistry, Molecular Biology and Biophysics,University of Minnesota, 3Institute on the Biology of Aging and Metabolism,University of Minnesota

Chapters

  • 00:04Title
  • 01:06Generation of Senescent Murine Embryonic Fibroblast (MEF) 12-15 Days
  • 03:46Senescent Associated -Galactosidase (SA–Gal) Screening Assay 2-3 Days
  • 06:06Results: Identification of Senotherapeutics by Measuring SA–Gal Activity in Single Cells
  • 06:48Conclusion

Summary

Automatic Translation

Automatic Translation

细胞衰老是慢性年龄相关疾病发展的关键因素。针对衰老细胞的治疗药物的鉴定为延长健康衰老提供了希望。在这里,我们提出了一个新的测定,以筛选基于测量与衰老相关的β-加拉托西沙酶在单细胞中的异质疗法的识别。

Tags

SA-β-GalactosidaseSenotherapeutic DrugsSenolyticSenomorphicHigh-throughput Drug ScreeningAging-related DiseasesCell SenescenceCell CultureTrypsinization

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