Journal
/
Cancer Research
/
Live-Cell Imaging Assays to Study Glioblastoma Brain Tumor Stem Cell Migration and Invasion
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal Cancer Research
Live-Cell Imaging Assays to Study Glioblastoma Brain Tumor Stem Cell Migration and Invasion

Live-Cell Imaging Assays to Study Glioblastoma Brain Tumor Stem Cell Migration and Invasion

DOI:
10.3791/58152-v

•

09:36 min

•

August 29, 2018

•

, , , ,
1Hotchkiss Brain Institute, Department of Cell Biology and Anatomy, Cumming School of Medicine,University of Calgary, 2Department of Developmental and Stem Cell Biology Program, Arthur and Sonia Labatt Brain Tumor Research Centre,The Hospital for Sick Children

Chapters

  • 00:04Title
  • 00:48Human Glioblastoma Specimen-derived Brain Tumor Stem Cell (BTSC) Culture
  • 02:40BTSC Migration Assay
  • 05:01BTSC Neurosphere Invasion Assay
  • 07:16Results: Representative BTSC Migration and Invasion Analyses
  • 09:00Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe live-cell imaging techniques to quantitatively measure the migration and invasion of glioblastoma brain tumor stem cells over time and under multiple treatment conditions.

Tags

Keywords: Live-cell ImagingGlioblastomaBrain Tumor Stem Cells (BTSCs)MigrationInvasionCancer Stem CellsCell CultureNeurosphereCell DetachmentChemotaxisDrug Treatment

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/58152?article_type=v

Coculture Assays to Study Macrophage and Microglia Stimulation of Glioblastoma Invasion
check_url/58152?article_type=v

Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models
check_url/58152?article_type=v

Long-term Live-cell Imaging to Assess Cell Fate in Response to Paclitaxel
check_url/58152?article_type=v

The Clinical Application of Tumor Treating Fields Therapy in Glioblastoma
check_url/58152?article_type=v

A Simple Migration/Invasion Workflow Using an Automated Live-cell Imager
check_url/58152?article_type=v

Longitudinal Intravital Imaging of Brain Tumor Cell Behavior in Response to an Invasive Surgical Biopsy
check_url/58152?article_type=v

Impedance-based Real-time Measurement of Cancer Cell Migration and Invasion
check_url/58152?article_type=v

Co-culture of Glioblastoma Stem-like Cells on Patterned Neurons to Study Migration and Cellular Interactions
check_url/58152?article_type=v

An Ex Vivo Brain Slice Model to Study and Target Breast Cancer Brain Metastatic Tumor Growth
check_url/58152?article_type=v

Real-Time Quantitative Measurement of Tumor Cell Migration and Invasion Following Synthetic mRNA Transfection

Read Article