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Journal / Biochemistry / 单分子力谱中蛋白质共价固定化的研究…
JoVE Journal
Biochemistry

This content is Open Access.

JoVE Journal Biochemistry
单分子力谱中蛋白质共价固定化的研究
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单分子力谱中蛋白质共价固定化的研究

Article DOI: 10.3791/58167-v • 11:13 min • August 20th, 2018
August 20th, 2018 •
1,2,3, 2, 1,3, 3, 2, *4, *1,3
1Center for Applied Tissue Engineering and Regenerative Medicine, Munich University of Applied Sciences, 2FG Protein Biochemistry & Cellular Microbiology, Munich University of Applied Sciences, 3Center for Nano Science, Ludwig-Maximilians-Universität München, 4Klinik für Unfallchirurgie, Orthopädie und Plastische Chirurgie, University Medical Center Göttingen
* These authors contributed equally

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本协议描述了 heterobifunctional 硅烷偶联剂在硅氧化物表面上的共价固定化, 其作用是以原子力显微镜为基础的单分子力光谱学为例。RrgA (pilus-1 尖黏附)与纤连蛋白的相互作用。

Tags

生物化学 问题 138 共价蛋白固定化 表面硅烷化 heterobifunctional PEG 试剂 亚胺基化学 初级氨基 原子力显微镜 AFM 单分子力光谱学 smf
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