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Journal / Biochemistry / Måling af Ion koncentration i Unstirred grænselag …
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Biochemistry

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JoVE Journal Biochemistry
Måling af Ion koncentration i Unstirred grænselag med åben Patch-Clamp Pipette
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Måling af Ion koncentration i Unstirred grænselag med åben Patch-Clamp Pipette: konsekvenser i kontrol af Ionkanaler af væske Flow

Article DOI: 10.3791/58228-v • 05:42 min • January 7th, 2019
January 7th, 2019 •
*1, *2, 1, 1, 3, 1
1Department of Physiology, KU Open Innovation Center, Research Institute of Medical Science, Konkuk University School of Medicine, 2Department of Emergency Medical Services, Eulji University, 3Department of Mechanical Engineering, Sungkyunkwan University
* These authors contributed equally

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Mechanosensitive Ionkanaler er ofte studerede i form af væske flow/shear kraft følsomhed med patch-clamp optagelse. Dog afhængig af forsøgsplan, kan resultatet på væske flow-forordninger af Ionkanaler være fejlagtig. Her, leverer vi metoder til at forebygge og rette sådanne fejl med et teoretisk grundlag.

Tags

Biokemi spørgsmålet 143 væskestrøm vride kraft unstirred lag patch-clamp Ag/AgCl referenceelektrode væske/metal junction potentiale konvektion ion-kanal
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