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Uracil-DNA-glycosylaseassay ved matrixassisteret laserdesorption/ionisering Time-of-flight Massespektrometrianalyse
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1Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine,National Taiwan University, 2Department of Laboratory Medicine,National Taiwan University Hospital, 3Center for Microbial Pathogenesis, Nationwide Children’s Hospital and the Department of Pediatrics,The Ohio State University
Chapters
- 00:04Introduction
- 01:28DNA Glycosylase Assay
- 04:43Transferring UDG Reaction Products to a Matrix Chip
- 05:33Setting up the Assay Parameters on the Mass Spectrometer
- 07:19Mass Spectrometer Operation, Viewing Mass Spectra, and Analyzing the Data
- 09:03Results: UDG Assay Using MALDI-TOF MS for AP Site Containing Product Analysis
- 11:01Conclusion
En ikke-mærket, ikke-radio-isotopisk metode til assay uracil-DNA-glycosylaseaktivitet blev udviklet ved anvendelse af MALDI-TOF massespektrometri til direkte apurinisk/ apyrimidinisk stedholdig produktanalyse. Assayet viste sig at være ret simpelt, specifikt, hurtigt og let at bruge til DNA-glycosylasemåling.
