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Environment
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西瓜与瓶装葫芦之间的同源和异种移植在冷反应微 rna 研究中的应用
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JoVE Journal
Environment
Generating Homo- and Heterografts Between Watermelon and Bottle Gourd for the Study of Cold-responsive MicroRNAs
Please note that all translations are automatically generated.
Click here for the English version.
西瓜与瓶装葫芦之间的同源和异种移植在冷反应微 rna 研究中的应用
DOI:
10.3791/58242-v
•
07:22 min
•
November 20, 2018
•
Lingping Wang*
1
,
Xinyi Wu*
1
,
Guojing Li
2
,
Xiaohua Wu
,
Dehui Qin
,
Ye Tao
,
Pei Xu
2
1
Institute of Vegetables
,
Zhejiang Academy of Agricultural Sciences
,
2
State Key Lab Breeding Base for Sustainable Control of Plant Pest and Disease
,
Zhejiang Academy of Agricultural Sciences
,
3
Institute of Horticulture
,
Zhejiang Academy of Agricultural Sciences
,
4
Shanghai Biozeron Biotechnology Co., Ltd
Chapters
00:04
Title
01:19
Seed Sterilization and Germination
02:09
Seedling Growth and Grafting
02:48
Post-grafting Management, Cold Treatment, and Sampling
03:45
Library Preparation and High-throughput Sequencing
05:20
Results: miRNA Up- and Down-regulated in Cold Treated Homografts and Heterografts
06:17
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文提出了一个详细的方案, 有效地制作西瓜和葫芦之间的同质和异种移植, 除了组织取样、数据生成和数据分析的方法, 用于研究冷反应微 rna。
Tags
Watermelon
Bottle Gourd
Grafting
Homo- And Heterografts
Cold Stress
MiRNA Response
Plant Grafting Technique
Seed Germination
Seedling Preparation
Grafting Procedure
Growth Chamber
Humidity Control
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