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ホモとスイカとひょうたんの同種および異種接ぎ木を生成する応答性マイクロ Rna の研究のため
JoVE Journal
Environment
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JoVE Journal
Environment
Generating Homo- and Heterografts Between Watermelon and Bottle Gourd for the Study of Cold-responsive MicroRNAs
Please note that all translations are automatically generated.
Click here for the English version.
ホモとスイカとひょうたんの同種および異種接ぎ木を生成する応答性マイクロ Rna の研究のため
DOI:
10.3791/58242-v
•
07:22 min
•
November 20, 2018
•
Lingping Wang*
1
,
Xinyi Wu*
1
,
Guojing Li
2
,
Xiaohua Wu
,
Dehui Qin
,
Ye Tao
,
Pei Xu
2
1
Institute of Vegetables
,
Zhejiang Academy of Agricultural Sciences
,
2
State Key Lab Breeding Base for Sustainable Control of Plant Pest and Disease
,
Zhejiang Academy of Agricultural Sciences
,
3
Institute of Horticulture
,
Zhejiang Academy of Agricultural Sciences
,
4
Shanghai Biozeron Biotechnology Co., Ltd
Chapters
00:04
Title
01:19
Seed Sterilization and Germination
02:09
Seedling Growth and Grafting
02:48
Post-grafting Management, Cold Treatment, and Sampling
03:45
Library Preparation and High-throughput Sequencing
05:20
Results: miRNA Up- and Down-regulated in Cold Treated Homografts and Heterografts
06:17
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
ホモとスイカと組織採取、データ生成、およびデータ解析の応答性マイクロ Rna の調査のためのメソッドに加えて、ひょうたんの同種および異種接ぎ木を効率的に作るための詳しいプロトコルをご紹介します。
Tags
Watermelon
Bottle Gourd
Grafting
Homo- And Heterografts
Cold Stress
MiRNA Response
Plant Grafting Technique
Seed Germination
Seedling Preparation
Grafting Procedure
Growth Chamber
Humidity Control
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