Contractions of Human-iPSC-derived Cardiomyocyte Syncytia Measured with a Ca-sensitive Fluorescent Dye in Temperature-controlled 384-well Plates

Camille Forny; Romain Sube; Eric A. Ertel

doi:10.3791/58290

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Contractions of Human-iPSC-derived Cardiomyocyte Syncytia Measured with a Ca-sensitive Fluorescent Dye in Temperature-controlled 384-well Plates

JoVE Journal
Medicine

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JoVE Journal Medicine

Contractions of Human-iPSC-derived Cardiomyocyte Syncytia Measured with a Ca-sensitive Fluorescent Dye in Temperature-controlled 384-well Plates

6,156 Views

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07:42 min

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October 18, 2018

DOI:

10.3791/58290-v

DOI:

10.3791/58290-v

•

07:42 min

October 18, 2018

•

5 Views

Camille Forny¹, Romain Sube¹, Eric A. Ertel¹

¹Department of Pharmacology and Preclinical Development,Idorsia Pharmaceuticals Ltd

Summary

Spontaneously contracting syncytia of cardiomyocytes derived from human-induced pluripotent stem cells are useful models of human cardiac physiology and pharmacology. Here we present a high-throughput screening system to quantify the effects of exogenous compounds on beating frequency, using a Ca-sensitive fluorescent dye and a temperature-controlled imaging multi-well plate reader.