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Visualisere Node og Notochordal plade i Gastrulating mus embryoner ved hjælp af Scanning elektronmikroskopi og hele Mount immunfluorescens
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1Graduate Program in Pharmacology and Experimental Therapeutics,University of Cologne, 2Department of Dermatology and Venereology,University Hospital of Cologne, 3Cologne Cluster of Excellence in Cellular Stress Responses in Aging-Associated Diseases (CECAD),University of Cologne, 4Department of General Ecology, Institute for Zoology, Biocenter Cologne,University of Cologne
Chapters
- 00:04Title
- 00:59Scanning Electron Microscopy of the Mouse Embryonic Node
- 04:17Whole Mount Immunofluorescence of the Mouse Node and Notochordal Plate
- 08:06Results: Strip1 Mutant Embryos Display Irregular Nodes and Abnormal F-Actin Organization in the Axial Mesoderm
- 08:46Conclusion
Node og notochordal plade er forbigående signalering arrangører udvikle mus embryoner, der kan visualiseres ved hjælp af flere teknikker. Her, vi beskriver i detaljer, hvordan du udfører to af teknikker til at studere deres struktur og morfogenese: 1) scanning elektronmikroskopi (SEM); og 2) hele mount immunofluorescens (WMIF).
