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Laser-assistert Lentiviral gen levering til musen befruktede egg
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Laser-assistert Lentiviral gen levering til musen befruktede egg

Article DOI: 10.3791/58327-v • 06:03 min • November 1st, 2018
November 1st, 2018 •
*1, *2, 1, 1, 1, 1, 1, 1, 3, 4, 1
1Neurobiology Laboratory, National Institute of Environmental Health Sciences, 2Comparative Medicine Branch, National Institute of Environmental Health Sciences, 3Reproductive and Developmental Biology Laboratory, National Institute of Environmental Health Sciences, 4Signal Transduction Laboratory, National Institute of Environmental Health Sciences
* These authors contributed equally

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Mus befruktede egg og tidlig stadium embryo er beskyttet av zona pellucida, en glykoprotein matrise som danner en barriere mot gen levering. Denne artikkelen beskriver en protokoll for perforating i zona med en laser transduce embryonale celler med lentiviral vektorer og opprette transgene mus.

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Biologi problemet 141 Transgenesis XYClone laser lentivirus mus befruktet egg mouse embryoer gen levering signaltransduksjon
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