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Lentiviralmediert levering av shRNA til hESC og NPC ved bruk av lavpris kationisk polymerpolyetylenimine (PEI)
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Lentiviralmediert levering av shRNA til hESC og NPC ved bruk av lavpris kationisk polymerpolyetylenimine (PEI)

Article DOI: 10.3791/63953-v • 06:49 min • May 24th, 2022
May 24th, 2022 •
1, 1,2
1Department of Biochemistry and Molecular Biology, College of Medicine and Health Sciences, United Arab Emirates University, 2Zayed Center for Health Sciences, United Arab Emirates University

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Ved hjelp av lavkost kationisk polymerpolyetylenimine (PEI) produserte vi lentivirale partikler for stabilt uttrykk av shRNA i H9 humane embryonale stamceller (hESCs) og forbigående transducerte H9-avledede nevrale stamceller (NPC) ved høy effektivitet.

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Biologi utgave 183 lentivirus shRNA hESCs NPCs polyetylenimine HEK293T celler
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