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1Neurobiology Laboratory,National Institute of Environmental Health Sciences, 2Comparative Medicine Branch,National Institute of Environmental Health Sciences, 3Reproductive and Developmental Biology Laboratory,National Institute of Environmental Health Sciences, 4Signal Transduction Laboratory,National Institute of Environmental Health Sciences
Chapters
- 00:04Title
- 00:55Mouse Fertilized Egg Isolation
- 02:12Mouse Fertilized Eggs XYClone Laser Perforation
- 03:13Mouse Fertilized Egg Transduction and Non-surgical Transfer
- 04:00Results: Representative Lentivirus Transduction Analyses
- 04:55Conclusion
Mouse di uova fecondate ed embrioni in fase precoce sono protetti dalla zona pellucida, una matrice di glicoproteina che forma una barriera contro la consegna del gene. Questo articolo descrive un protocollo per la zona di perforazione con un laser per trasdurre cellule embrionali con vettori lentivirali e creare topi transgenici.
