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1Neurobiology Laboratory,National Institute of Environmental Health Sciences, 2Comparative Medicine Branch,National Institute of Environmental Health Sciences, 3Reproductive and Developmental Biology Laboratory,National Institute of Environmental Health Sciences, 4Signal Transduction Laboratory,National Institute of Environmental Health Sciences
Chapters
- 00:04Title
- 00:55Mouse Fertilized Egg Isolation
- 02:12Mouse Fertilized Eggs XYClone Laser Perforation
- 03:13Mouse Fertilized Egg Transduction and Non-surgical Transfer
- 04:00Results: Representative Lentivirus Transduction Analyses
- 04:55Conclusion
Mus befruktade ägg och tidiga skede embryon skyddas av zona pellucida, ett glykoprotein matris som bildar en barriär mot gen leverans. I artikeln beskrivs ett protokoll för perforering av zona med laser transduce embryonala celler med lentiviral vektorer och skapa transgena möss.
