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寄生性锥虫锥虫锥虫的 f1-atpase 的分离
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Biochemistry
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JoVE Journal Biochemistry
Isolation of F1-ATPase from the Parasitic Protist Trypanosoma brucei
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寄生性锥虫锥虫锥虫的 f1-atpase 的分离

DOI:
10.3791/58334-v

•

08:44 min

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January 22, 2019

•

,
1Biology Centre,Czech Academy of Science, Institute of Parasitology, 2Faculty of Science,University of South Bohemia

Chapters

  • 00:04Title
  • 00:57Preparation of Sub-Mitochondrial Particles
  • 02:43Chloroform Release of F1-ATPase from the Membrane
  • 04:08Anion-Exchange Chromatography
  • 05:34Size-Exclusion Chromatography
  • 06:49Results: Two-Step Purification Results in Effective Isolation of F1-ATPase
  • 08:03Conclusion

Summary

Automatic Translation

Automatic Translation

该方案描述了从锥虫培养阶段的 f1-atpase 的纯化.该程序产生了高度纯、均匀和活性的复合物, 适用于结构和酶学研究。

Tags

F1-ATPaseTrypanosoma BruceiMitoplastsProtein PurificationMitochondrial MembraneSonicationUltracentrifugationChloroform ExtractionDounce Homogenizer

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