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Isolering av F-1-ATPase fra den parasittiske Protist Trypanosoma brucei
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Isolation of F1-ATPase from the Parasitic Protist Trypanosoma brucei
DOI:

08:44 min

January 22, 2019

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Chapters

  • 00:04Title
  • 00:57Preparation of Sub-Mitochondrial Particles
  • 02:43Chloroform Release of F1-ATPase from the Membrane
  • 04:08Anion-Exchange Chromatography
  • 05:34Size-Exclusion Chromatography
  • 06:49Results: Two-Step Purification Results in Effective Isolation of F1-ATPase
  • 08:03Conclusion

Summary

Automatic Translation

Denne protokollen beskriver rensing av F-1-ATPase kulturperler insekt scenen Trypanosoma brucei. Prosedyren gir en svært ren, homogen og aktive kompleks egnet for strukturelle og enzymatiske studier.

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