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1Lab of Animal Models and Functional Genomics (LAMFG), The Key Laboratory of Animal Vaccine & Protein Engineering, College of Veterinary Medicine,Hunan Agricultural University (HUNAU), 2Department of Pathology,Georgetown University Medical School, 3College of Food Science and Technology,Hunan Agricultural University (HUNAU), 4Lab of Molecular Cardiology (LMC),National Heart, Lung, and Blood Institute (NHLBI)/National Institutes of Health (NIH), 5Transgenic Core,National Heart, Lung, and Blood Institute (NHLBI)/National Institutes of Health (NIH)
Chapters
- 00:04Title
- 01:02Southern Blotting Screening
- 04:40Results: Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells
- 06:07Conclusion
Aquí, presentamos un protocolo detallado para la identificación de eventos de recombinación homóloga que se produjeron en células madre embrionarias del ratón mediante Southern blot o PCR. Este método es ejemplificado por la generación de modelos UROLOGICAL miosina II reemplazo genético ratón tecnología tradicional embrionario basada en células madre homólogo mediada por la recombinación dirigida a.
