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蛋白质抗原对羊的免疫和抗原特异性单域抗体的制备
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Immunization of Alpacas (Lama pacos) with Protein Antigens and Production of Antigen-specific Single Domain Antibodies
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蛋白质抗原对羊的免疫和抗原特异性单域抗体的制备

DOI:
10.3791/58471-v

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05:27 min

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January 26, 2019

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, , , , , , , ,
1Department of Molecular and Cellular Biochemistry,University of Kentucky, 2Division of Laboratory Animal Resources,University of Kentucky, 3River Hill Ranch

Chapters

  • 00:04Title
  • 01:39Generation of Antigen-specific Alpaca Antibodies
  • 03:02Purifying Lymphocytes for Single Domain Antibody Library Construction
  • 03:59Results: Single Domain Antibodies Generated against a Range of Protein Antigens in Alpacas (Lama pacos)
  • 04:46Conclusion

Summary

Automatic Translation

Automatic Translation

介绍了一种从羊的免疫、血液采集、b 细胞分离和选择等方面制备单域抗体的方法。

Tags

AlpacaImmunizationProtein AntigensSingle Domain AntibodiesBiomedical ResearchCamelidsAntigen InjectionBlood CollectionHandlingVeterinary Techniques

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