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Cancer Research
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从小鼠的补丁中提取体液免疫的关键参与者: 先进和优化的淋巴细胞分离协议
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Unraveling Key Players of Humoral Immunity: Advanced and Optimized Lymphocyte Isolation Protocol from Murine Peyer’s Patches
Please note that all translations are automatically generated.
Click here for the English version.
从小鼠的补丁中提取体液免疫的关键参与者: 先进和优化的淋巴细胞分离协议
DOI:
10.3791/58490-v
•
08:25 min
•
November 21, 2018
•
Yavuz F. Yazicioglu
2
,
Halil I. Aksoylar
2
,
Rinku Pal
2
,
Nikolaos Patsoukis
2
,
Vassiliki A. Boussiotis
2
1
Division of Hematology-Oncology, Beth Israel Deaconess Medical Center
,
Harvard Medical School
,
2
Department of Medicine, Beth Israel Deaconess Medical Center
,
Harvard Medical School
Chapters
00:04
Title
00:40
Peyer’s Patch (PP) Harvest and Single Cell Suspension Preparation
04:08
Surface Marker and Intracellular Protein Staining
06:23
Results: Representative Isolated PP Lymphocyte Population Analyses
08:04
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这项研究中, 我们提出了一个新的和有效的方案, 从 peyer 的补丁 (pp) 的淋巴细胞分离, 随后可用于
体内
和
体外
功能分析, 以及流式细胞仪研究的卵泡 t辅助和萌发中心 b 细胞。
Tags
Lymphocyte Isolation
Peyer’s Patches
Murine
T Cells
B Cells
Follicular T Helper Cells
Germinal Center B Cells
Cell Isolation Protocol
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