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Biochemistry
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利用单击化学辅助 rna-路特捕获 (无花果) 策略捕获和鉴定 rna 结合蛋白
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Biochemistry
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JoVE Journal
Biochemistry
Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture (CARIC) Strategy
Please note that all translations are automatically generated.
Click here for the English version.
利用单击化学辅助 rna-路特捕获 (无花果) 策略捕获和鉴定 rna 结合蛋白
DOI:
10.3791/58580-v
•
09:36 min
•
October 19, 2018
•
Rongbing Huang*
1,2
,
Mengting Han*
1,2
,
Liying Meng
3
,
Xing Chen
2,3,4,5
1
College of Chemistry and Molecular Engineering
,
Peking University
,
2
Beijing National Laboratory for Molecular Sciences
,
Peking University
,
3
Peking-Tsinghua Center for Life Sciences
,
Peking University
,
4
Synthetic and Functional Biomolecules Center
,
Peking University
,
5
Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education
,
Peking University
Chapters
00:04
Title
00:38
Preparation of Lysate of Metabolically Labeled and UV Cross-linked Cells
03:33
Preparation of Samples for RNA-interactome Capture
05:13
RNA-interactome Capture
07:45
Results: Quality Control of Captured RNA-binding Proteins
08:53
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文介绍了应用单击化学辅助 RNA-路特捕获 (无花果) 策略来识别与编码和非编码 rna 结合的蛋白质的详细协议。
Tags
RNA-binding Proteins
CARIC
Click Chemistry
RNA-interactome Capture
Post-transcriptional Gene Regulation
MRNA
Non-coding RNA
HeLa Cells
EU
4SU
UV Cross-linking
Cell Lysis
RNA-protein Interaction
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