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Biochemistry

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JoVE Journal Biochemistry
Hvordan kvantifisere brøkdel av Photoactivated fluorescerende proteiner i Bulk og lever celler
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Hvordan kvantifisere brøkdel av Photoactivated fluorescerende proteiner i Bulk og lever celler

Article DOI: 10.3791/58588-v • 11:03 min • January 7th, 2019
January 7th, 2019 •
1, 1
1Gynecologic Oncology Division, Stanford University School of Medicine

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Her presenterer vi en protokoll som innebærer genetisk kombinert spectrally forskjellige photoactivatable og fluorescerende proteiner. Disse fluorescerende protein chimeras tillater kvantifisering av PA-FP brøken som photoactivated skal fluorescerende, dvs photoactivation effektivitet. Protokollen avslører at forskjellige moduser av photoactivation gir forskjellige photoactivation effektivitet.

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Biokjemi problemet 143 Photoactivatable fluorescerende proteiner photoactivation effektivitet ratiometric intensitetsbasert ensemble studier photoconvertible fluorescerende proteiner GFP mCherry
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