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在暴露于臭氧的小鼠的弹性周期中的肺微 rna 分析
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Immunology and Infection
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JoVE Journal Immunology and Infection
Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice
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在暴露于臭氧的小鼠的弹性周期中的肺微 rna 分析

DOI:
10.3791/58664-v

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07:07 min

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January 07, 2019

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1Pulmonary, Immunology and Physiology Laboratory, Department of Pediatrics,Pennsylvania State University College of Medicine, 2Department of Biochemistry and Molecular Biology,Pennsylvania State University College of Medicine

Chapters

  • 00:04Title
  • 00:31Estrous Cycle Stage Assessment, Ozone Exposure, and Lung Collection
  • 02:11RNA Preparation
  • 03:43microRNA (miRNA) Profiling
  • 04:40Results: Representative Functional microRNA Analyses
  • 06:26Conclusion

Summary

Automatic Translation

Automatic Translation

在这里, 我们描述了一种方法来评估肺部表达 mirna, 预测调节炎症基因使用小鼠暴露在臭氧或过滤空气在不同阶段的雌周期。

Tags

MicroRNALungEstrous CycleOzoneMouseInflammationVaginal FluidRNA ExtractionReal-time PCR

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