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基于 crispr-cas9 的竞争检测对遗传依赖性的调查
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Investigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays
DOI:

11:05 min

January 07, 2019

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Chapters

  • 00:04Title
  • 00:30Medium-throughput Cloning of sgRNAs
  • 04:55Viral Production of sgRNA Constructs and Transduction in AML-Cas9 Cells
  • 07:10Competitive Growth Assay
  • 09:21Results: The Role of Candidate Genes in AML Cell Proliferation
  • 10:30Conclusion

Summary

Automatic Translation

这份手稿描述了一种基于群集的规则间的短半突 (crispr) crispr-cas9 方法, 用于简单而快速地调查多个候选基因在急性髓样白血病 (aml) 细胞增殖中的作用。并行。这种技术是可扩展的, 也可以应用于其他癌症细胞系。

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