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基于 crispr-cas9 的竞争检测对遗传依赖性的调查

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Cancer Research

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JoVE Journal Cancer Research

Investigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays

Please note that all translations are automatically generated. Click here for the English version.

基于 crispr-cas9 的竞争检测对遗传依赖性的调查

DOI:

10.3791/58710-v

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11:05 min

•

January 07, 2019

•

Anagha Deshpande, Bo Rui Chen, Luyi Zhao, Kelsey Saddoris, Mayuri Kerr, Nan Zhu, Prashant Mali, Aniruddha J. Deshpande

¹Tumor Initiation and Maintenance Program,Sanford Burnham Prebys Medical Discovery Institute, ²Blood Research Institute,Blood Center of Wisconsin, ³Department of Bioengineering,University of California, San Diego

Chapters

00:04Title
00:30Medium-throughput Cloning of sgRNAs
04:55Viral Production of sgRNA Constructs and Transduction in AML-Cas9 Cells
07:10Competitive Growth Assay
09:21Results: The Role of Candidate Genes in AML Cell Proliferation
10:30Conclusion

Summary

Automatic Translation

这份手稿描述了一种基于群集的规则间的短半突 (crispr) crispr-cas9 方法, 用于简单而快速地调查多个候选基因在急性髓样白血病 (aml) 细胞增殖中的作用。并行。这种技术是可扩展的, 也可以应用于其他癌症细胞系。

Tags

CRISPR-Cas9 Competition Assays Genetic Dependencies AML Single Guide RNA Oligonucleotides Annealing Ligation Vector Linearization High-throughput Screening

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