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新鲜分离和内联小鼠脑内皮细胞内钙和膜电位的同时测量
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Simultaneous Measurements of Intracellular Calcium and Membrane Potential in Freshly Isolated and Intact Mouse Cerebral Endothelium
Please note that all translations are automatically generated.
Click here for the English version.
新鲜分离和内联小鼠脑内皮细胞内钙和膜电位的同时测量
DOI:
10.3791/58832-v
•
09:45 min
•
January 20, 2019
•
Md A. Hakim
,
Erik J. Behringer
1
Department of Basic Sciences
,
Loma Linda University
Chapters
00:04
Title
00:48
Dissection and Isolation of Cerebral Artery
02:34
Preparation of Endothelial Tube and Superfusion
05:44
Simultaneous Measurement of [Ca
2+
]
i
and V
m
08:36
Results: Simultaneous [Ca
2+
]
i
and V
m
Recordings
09:10
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这里演示的是 (1) 新鲜隔离完整的大脑内皮 "管" 和 (2) 同时测量内皮钙和膜电位的协议, 在内皮衍生的超极化过程中。此外, 这些方法允许药理调节内皮细胞钙和电信号作为个人或交互式实验变量。
Tags
Intracellular Calcium
Membrane Potential
Cerebral Endothelium
Cerebral Vascular Physiology
Cerebral Blood Flow Regulation
Aging
Chronic Diseases
Mouse Brain Isolation
Cerebral Artery Isolation
Enzymatic Digestion
Simultaneous Measurement
Physiological Conditions
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