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利用可活性可选位的激光光致发光体研究小鼠脑片中的烟碱乙酰胆碱受体功能

JoVE Journal
Neuroscience

This content is Free Access.

JoVE Journal Neuroscience

Probing Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices via Laser Flash Photolysis of Photoactivatable Nicotine

Please note that all translations are automatically generated. Click here for the English version.

利用可活性可选位的激光光致发光体研究小鼠脑片中的烟碱乙酰胆碱受体功能

DOI:

10.3791/58873-v

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10:48 min

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January 25, 2019

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Matthew C. Arvin, David L. Wokosin, Sambashiva Banala, Luke D. Lavis, Ryan M. Drenan

¹Department of Pharmacology,Northwestern University Feinberg School of Medicine, ²Department of Physiology,Northwestern University Feinberg School of Medicine, ³Janelia Research Campus,Howard Hughes Medical Institute

Chapters

00:04Title
00:39Preparation of Photoactivatable Nicotine (PA-Nic)
03:23Imaging Neurons with 2-Photon Laser Scanning Microscopy
05:28Calibration of Uncaging Laser Galvanometers and Pairing of PA-Nic Laser Flash Photolysis with Electrophysiological Recordings
07:47Results: Representative 2-Photon Laser Scanning Microscopy Imaging and PA-Nic Laser Flash Photolysis Responses
09:47Conclusion

Summary

Automatic Translation

本文提出了一种用烟碱不加污染的方法研究小鼠脑片中烟碱乙酰胆碱受体 (nachr)。当与同时膜片钳记录和 2-光子激光扫描显微镜, 尼古丁发现连接烟碱受体功能与细胞形态, 提供了更深入的了解胆碱能神经生物学。

Tags

Keywords: Laser Flash Photolysis Photoactivatable Nicotine Nicotinic Acetylcholine Receptors Mouse Brain Slices Multi-photon Laser-scanning Microscopy Spatiotemporal Control Cholinergic Synaptic Transmission Volume Transmission Patch Clamp Pressure Ejection Bath Application Perfusion System

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