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高通量硝基苯并二氮唑标记的胆固醇积液测定
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Medicine
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JoVE Journal
Medicine
High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay
Please note that all translations are automatically generated.
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高通量硝基苯并二氮唑标记的胆固醇积液测定
DOI:
10.3791/58891-v
•
08:18 min
•
January 07, 2019
•
Roque Pastor-Ibáñez
,
Mireia Arnedo
,
Olivia Tort
1
Group of Genomics and Pharmacogenomics in HIV, Laboratory of Retrovirology and Viral Immunopathogenesis, IDIBAPS
,
Hospital Clínic de Barcelona
Chapters
00:04
Title
01:05
NBD-cholesterol Preparation, Cell Culture, and Seeding
02:20
Apolipoprotein B Depleted Serum (ABDS) Preparation
03:14
NBD-cholesterol Cell Loading and Incubation with Cholesterol Acceptors
04:29
Fluorescent Signal Capture
05:35
Results: Analysis of Cholesterol Efflux Assay
07:24
Conclusion
Summary
Automatic Translation
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Automatic Translation
在巨噬细胞模型中测量血清或血浆的体外胆固醇流出能力是动脉粥样硬化的一个很有前途的工具。在本研究中, 我们优化和规范了荧光 nbd-胆固醇外排法, 并开发了一个使用96孔板的高通量分析。
Tags
Cholesterol Efflux
NBD Cholesterol
THP-1 Cells
PEG Precipitation
Cardiovascular Risk
Cell-based Assay
High-throughput Screening
Lipid Metabolism
Macrophages
Stem Cells
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