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原代小鼠骨骼肌微血管内皮细胞的分离
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Neuroscience
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JoVE Journal Neuroscience
Isolation of Primary Murine Skeletal Muscle Microvascular Endothelial Cells
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原代小鼠骨骼肌微血管内皮细胞的分离

DOI:
10.3791/58901-v

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11:57 min

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March 06, 2019

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, , , ,
1Institute for Translational Neurology and Neurology Clinic,University of Muenster

Chapters

  • 00:04Title
  • 00:47Isolation of Primary Murine MMEC
  • 08:03Primary Murine MMEC Purification
  • 09:04Results: Analysis of the Isolated Primary Murine Skeletal Muscle Microvascular Endothelial Cells
  • 11:20Conclusion

Summary

Automatic Translation

Automatic Translation

骨骼肌微血管内皮细胞 (mmec) 塑造了肌肉毛细血管的内壁, 并调节两者, 流体分子的交换和 (免疫) 细胞在肌肉组织和血液之间的迁移。分离原生小鼠 mmeec, 如下所述, 使 "我的血管单位" 能够进行全面的体外调查。

Tags

Primary MurineSkeletal MuscleMicrovascular Endothelial CellsIsolationCell CultureDigestion

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