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JoVE Journal
Neuroscience

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Isolation of Primary Murine Brain Microvascular Endothelial Cells
 

Isolation of Primary Murine Brain Microvascular Endothelial Cells

Article doi: 10.3791/52204
November 14th, 2014

Summary November 14th, 2014

Brain microvascular endothelial cells (BMEC) are interconnected by specific junctional proteins forming a highly regulated barrier separating blood and the central nervous system (CNS), the so-called blood-brain-barrier (BBB). The isolation of primary murine brain microvascular endothelial cells, as discussed in this protocol, enables detailed in vitro studies of the BBB.

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