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人类多能干细胞有效分化为肝细胞

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Developmental Biology

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JoVE Journal Developmental Biology

Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells

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人类多能干细胞有效分化为肝细胞

DOI:

10.3791/58975-v

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07:37 min

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June 11, 2019

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Kyle M. Loh², Amrita Palaria, Lay Teng Ang

¹Institute for Stem Cell Biology & Regenerative Medicine, Stanford-UC Berkeley Siebel Stem Cell Institute,Stanford University School of Medicine, ²Department of Developmental Biology,Stanford University School of Medicine

Chapters

00:04Title
00:45Preparation of Differentiation Media
02:04Seed hPSCs onto Plates at Defined Densities for Differentiation
04:39Differentiation of hPSCs into Endodermal Cells and Liver Progenitors
05:37Results: Differentiating Human Pluripotent Stem Cells into Liver Cells
06:58Conclusion

Summary

Automatic Translation

该协议详细介绍了单层无血清方法,在18天内从人类多能干细胞(hPSCs)中高效生成肝细胞样细胞。这需要六个步骤,因为hPSCs在形成肝细胞样细胞之前,按顺序分化成中间细胞类型,如原始条纹、最终内皮、后前体和肝芽祖细胞。

Tags

Human Pluripotent Stem Cells Liver Cells Differentiation Regenerative Medicine Liver Bud Progenitors Hepatocyte-like Cells Developmental Signaling Pathways High Purity IMDM PVA CDM2 CDM3 CDM4 CDM5 Small Molecules Growth Factors Matrix HPSC Dissociation Agent

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