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一种评价金黄色葡萄球菌感染先天免疫反应的小鼠模型
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Mouse Model to Assess Innate Immune Response to Staphylococcus aureus Infection
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一种评价金黄色葡萄球菌感染先天免疫反应的小鼠模型

DOI:
10.3791/59015-v

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09:15 min

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February 28, 2019

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, , , ,
1Department of Biomedical Engineering,University of California Davis, 2Department of Dermatology,Johns Hopkins University School of Medicine

Chapters

  • 00:04Title
  • 00:38Bacterial Preparation and Quantification
  • 02:44Excisional Skin Wounding
  • 03:50Staphylococcus aureus Inoculation
  • 04:44In Vivo Bioluminescent and Fluorescence Imaging
  • 06:02Bioluminescent and Fluorescence Image Analysis
  • 06:45Results: LysM-EGFPMyD88-/- Mice are More Susceptible to S. aureus Infection Than LysM-EGFP Mice
  • 08:30Conclusion

Summary

Automatic Translation

Automatic Translation

介绍了一种实时检测小鼠皮肤损伤和金黄色葡萄球菌感染的先天免疫反应的方法。通过将 lysm-egfp 小鼠 (具有荧光中性粒细胞) 与 lysm-egfp 杂交免疫缺陷小鼠株进行比较, 提高了对感染的认识, 并提出了对抗感染的方法。

Tags

Mouse ModelStaphylococcus Aureus InfectionInnate Immune ResponseBioluminescenceBacterial BurdenNeutrophil ResponseLongitudinal MonitoringExcisional Skin WoundingBacterial InoculationColony-forming Units

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