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Biochemistry
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培养细胞功能化纳米化对跨 golgi 网络的内分泌吸收和逆行迁移分析
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Biochemistry
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JoVE Journal
Biochemistry
Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network Using Functionalized Nanobodies in Cultured Cells
Please note that all translations are automatically generated.
Click here for the English version.
培养细胞功能化纳米化对跨 golgi 网络的内分泌吸收和逆行迁移分析
DOI:
10.3791/59111-v
•
11:05 min
•
February 21, 2019
•
Dominik P. Buser
,
Martin Spiess
1
Biozentrum
,
University of Basel
Chapters
00:04
Title
01:03
Bacterial Liquid Culture and Induction of Functionalized Nanobody Expression
02:31
Purification of Functionalized Nanobodies
04:14
Validation of Functionalized Nanobody Expression
04:41
Uptake of Functionalized Nanobodies by Cultured Cells
08:03
Results: Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network
10:09
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
蛋白质从细胞表面逆行运输到 golgi 是维持膜稳态所必需的。在这里, 我们描述了一种方法, 生物化学分析细胞地对golgi 的重组蛋白运输使用功能化纳米棒在海拉细胞。
Tags
Nanobody
Endocytic Uptake
Retrograde Transport
Trans-Golgi Network
Cell Surface Receptors
Fluorescence Microscopy
Electron Microscopy
Tyrosine Sulfation
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