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Analyse af Endocytic optagelse og retrograd Transport til Trans-Golgi netværket ved hjælp af Functionalized Nanobodies i kulturperler celler
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Chapters
- 00:04Title
- 01:03Bacterial Liquid Culture and Induction of Functionalized Nanobody Expression
- 02:31Purification of Functionalized Nanobodies
- 04:14Validation of Functionalized Nanobody Expression
- 04:41Uptake of Functionalized Nanobodies by Cultured Cells
- 08:03Results: Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network
- 10:09Conclusion
Retrograd transport af proteiner fra cellens overflade til Golgi er afgørende at fastholde membran homøostase. Her, beskriver vi en metode til at analysere biokemisk celle overflade til Golgi transport af rekombinante proteiner ved hjælp af functionalized nanobodies i HeLa celler.
