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Agrobacterium tumefaciens og Agrobacterium rhizogenes-medieret Transformation af kartoffel og promotor aktivitet af en Suberin gen af GUS Staining
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Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining
DOI:

08:31 min

March 29, 2019

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Chapters

  • 00:04Title
  • 00:44Agrobacterium Culture Preparation
  • 01:46Plant Transformation Using A. rhizogenes
  • 03:19Plant Transformation using A. tumefaciens
  • 05:28Beta-glucuronidase (GUS) Histochemical Reporter Gene Assay
  • 06:20Results: Representative Imaging of Transformed Potato Roots
  • 07:30Conclusion

Summary

Automatic Translation

Vi præsenterer her, to protokoller for at omdanne kartoffelplanter. Agrobacterium tumefaciens transformation fører til et komplet transgene anlæg mens Agrobacterium rhizogenes producerer transgene behårede rødder i en vildtype skyde, der kan være selvstændige opformeret. Vi registrerer promotor aktivitet af GUS farvning i de transformerede rødder.

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