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Cancer Research
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全组织细胞外囊的提取
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Extraction of Extracellular Vesicles from Whole Tissue
Please note that all translations are automatically generated.
Click here for the English version.
全组织细胞外囊的提取
DOI:
10.3791/59143-v
•
09:03 min
•
February 07, 2019
•
Stephanie N. Hurwitz
,
James M. Olcese
,
David G. Meckes Jr.
1
Department of Biomedical Sciences
,
Florida State University College of Medicine
Chapters
00:04
Title
00:51
Tissue Dissociation and Differential Centrifugation
03:15
Density Gradient Purification
05:53
Lysis and Immunoblot Confirmation of EV Proteins
07:10
Results: Analysis of Extracted Extracellular Vesicles
08:09
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提供了一个详细的方案, 以分离细胞外囊泡 (ev) 从整个组织, 包括大脑和肿瘤标本。该方法提供了一种可重复的技术, 可从固体组织中提取 ev, 以便进一步进行下游分析。
Tags
Extracellular Vesicles
Ultracentrifugation
EV Isolation
Tumor Specimens
Protease Inhibitors
Centrifugation
Filtration
Resuspension
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